Table 2.
Growth range, halotolerance, and molecular identification of the isolated yeasts used on the resistance experiments, using BLASTn and ITS region
| Isolate ID | Sample ID | Primer | Result top BLAST (access no. GenBank) | Identity (%) | No. of bp analyzed | Species or proposed taxonomic group (GenBank access no.) | Halotolerance (NaCl) | Max. temperature with observable growth | Min. temperature with observable growth |
|---|---|---|---|---|---|---|---|---|---|
| 16Lv2 | S5047 | NL | Cryptococcus friedmannii (JX092255) | 100 | 599 | Cryptococcus friedmannii (KM243310) | 1.75 mol/L | 25°C (weak) | −6.5°C |
| ITS | Cryptococcus friedmannii (AF145322) | 99 | 549 | Cryptococcu sfriedmannii (KM243311) | |||||
| 15Lv1 | S4823 | NL | Exophiala capensis (JF499861) | 99 | 587 | Exophiala sp. (KM243304) | 1.25 mol/L | 25°C | 0°C |
| ITS | Exophiala sp. (HQ452332) | 99 | 509 | Exophiala sp. (KM243305) | |||||
| 16Lv1 | S3981 | NL | Holtermanniella watticus (KC006859) | 99 | 601 | Holtermanniella watticus (KM243308) | 2.25 mol/L | 25°C (weak) | −6.5°C |
| ITS | Holtermanniella watticus (JQ857031) | 99 | 501 | Holtermanniella watticus (KM243309) | |||||
| 16Lv3 | S3981 | NL | Rhodosporidium toruloides (GQ169736) | 99 | 587 | Rhodosporidium toruloides (KM243312) | 0.75 mol/L | 30°C | 10°C |
| ITS | Rhodosporidium toruloides (JN246549) | 98 | 524 | Rhodosporidium toruloides (KM243313) |
ITS, internal transcribed spacer.
1
Coverage of 100% for all sequences analyzed.