Table 1.
Purification of FCVC
| Step | Total protein (mg) | Total activity1 (U) | Specific activity (U/mg) | Yield (%) |
|---|---|---|---|---|
| Cell extract | 710 | 64,000 | 90 | 100 |
| Ultracentrifugation | 534 | 62,080 | 116 | 97 |
| Salting out (30–50%) | 207 | 36,800 | 178 | 58 |
| Anion exchange chromatography (UNO Q-6) | 7.6 | 11,520 | 1518 | 18 |
| Hydroxyapatite chromatography (CHT2-1) | 0.54 | 3840 | 7111 | 6 |
| First gel filtration chromatography (SD200 10/300 GL) | 0.02 | 1280 | 64,000 | 2 |
| Second gel filtration chromatography (SD200 10/300 GL) | 0.001 | 80 | 80,000 | 0.125 |
1
The activity of FCVC to convert VBNC Vibrio cholerae to a culturable state was measured as described in the text.