Table 1.
List of strains and plasmids used
| Strains or plasmids | Relevant properties | Source or reference |
|---|---|---|
| Escherichia coli | ||
| JM109 | recA1, endA1, gyrA96, thi, hsdR17(rk− mk+), e14−(mcrA−), supE44, relA1, Δ(lac-proAB)/F’[traD36, proAB+, lacIq, lacZΔM15] | Laboratory strain |
| MLA301 | MG1655 alr::FRT, dadX::FRT, yfdZ::FRT, avtA::GM, yfbQ::KM | Hori et al. (2001a) |
| MLA301ΔalaE | MLA301 derivative with a deletion in the alaE gene | Hori et al. (2001b) |
| MG1655 | wild-type | Hori et al. (2001b) |
| MG1655ΔalaE | MG1655 derivative with a deletion in the alaE gene | Hori et al. (2001b) |
| Plasmid | ||
| pSTV29 | CPr, lacZ, p15A ori | Takara |
| pAlaE | pSTV29 harboring a 1.0-kb PCR fragment of the alaE gene | Hori et al., (2011b) |
GM, gentamicin; KM, kanamycin; CP, chloramphenicol; AP, ampicillin.