Table 1.
Strains and plasmids in this study
| Strains or plasmids | Relevant characteristics | References or origins |
|---|---|---|
| Strains | ||
| Topo10 | A cloning Escherichia coli host (F−, ΔlacX74) | Invitrogen |
| BL21(DE3) | An expression E. coli host | Lab stock |
| FYJ179 | Agrobacterium tumefaciens NTL4 | Feng et al. (2013b) |
| FYJ284 | NTL4, ΔbioR::Km, ΔbioBFDA | Feng et al. (2013a,b) |
| FYJ291 | FYJ284 (NTL4, ΔbioR::Km, ΔbioBFDA) carrying pRG-PbioBat | Feng et al. (2013a,b) |
| PD1222 | The wild-type strain of Paracoccus denitrificans | ATCC |
| FYJ347 | Topo carrying pET28-bioRpd1 | This work |
| FYJ350 | Topo carrying pET28-bioRpd2 | This work |
| FYJ351 | BL21 (DE3) carrying pET28-bioRpd1 | This work |
| FYJ354 | BL21 (DE3) carrying pET28-bioRpd2 | This work |
| FYJ376 | FYJ291 carrying pSRKGm-bioRpd1 | This work |
| FYJ377 | FYJ291 carrying pSRKGm-bioRpd2 | This work |
| Plasmids | ||
| pET28(a) | Commercial T7-driven expression vector, KmR | Novagen |
| pET28- bioRpd1 | pET28(a) carrying P. denitrificans bioRpd1 gene, KmR | This work |
| pET28- bioRpd2 | pET28(a) carrying P. denitrificans bioRpd2 gene, KmR | This work |
| pSRKGm | Broad host range expression vector with the tightly regulated promoter | Feng et al. (2013b) |
| pSRK-bioRpd1 | pSRKGm encoding P. denitrificans bioRpd1 gene, GmR | This work |
| pSRK-bioRpd2 | pSRKGm encoding P. denitrificans bioRpd2 gene, GmR | This work |
| pRG970 | Low copy transcriptional promoter-less lacZ/Gus bi-directional fusion vector, SpcR | Van den Eede et al. (1992); van Dillewijn et al. (2001) |
| pRG-PbioBat | pRG970 encoding the A. tumefaciens bioBFDAZ promoter region | Feng et al. (2013a,b) |
ATCC, American Type Culture Collection.