Table 1.
List of Caenorhabditis elegans and bacterial strains, and plasmids used in this study
| Strain | Description | Reference or source |
|---|---|---|
| Escherichia coli | ||
| DH5α | F’ endA1 hsdR17(rk- mk-) supE44 thi-1 recA1 gyrA (Nalr) relA1 Δ(lacZYA-argF)U169 deoR(ɸ80lacΔ(lacZ)M15) | New England Biolabs |
| DH5αλpir | K-12 F- φ80lacZΔM15 endA recAhsdR17 (rm- mK+)supE44 thi-1 gyrA96 relA1 Δ(lacZYA-argF) U169 λpir | M. Swanson (Bryan et al. 2013) |
| OP50 | Uracil auxotroph | CGC (Brenner 1974) |
| HT115(DE3) | rnc14::ΔTn10λ(DE3) | J. Kinchen (Timmons et al. 2001) |
| MP375 | HT115 pL4440::ced-3, Ampr Tetr | This study |
| unc-22 RNAi | HT115 pL4440::rme-2, Ampr Tetr | Arhinger RNAi library (ZK617.1 [F03 IV]) |
| Legionella pneumophila | ||
| Lp02 | Strr, Thy−, HsdR− derivative of Philadelphia-1 strain | M. Swanson (Berger and Isberg 1993) |
| Lp03 | Lp02 dotA03 thyA− | R. Isberg (Berger and Isberg 1993) |
| Lp03 comp | Lp03 pKB9 | R. Isberg (Roy et al. 1998) |
| Lp02 ΔsdhA | Deletion of sdhA | R. Isberg (Laguna et al. 2006) |
| Lp02 ΔsdhA comp | Lp02 pRL101 | R. Isberg (Laguna et al. 2006) |
| KB421 | Lp02 ΔmagA::gfpmut3 | This study |
| Lp02 thyA comp | Lp02 pBH6119 | |
| Plasmids | ||
| pSR47s | oriTRP4 oriR6K kan sacB suicide vector | J. Vogel (Merriam et al. 1997) |
| pSK | Cloning vector, Ampr | Stratagene |
| pKB127 | pBH6119::PmagA 245-bp region cloned into BamHI and XbaI sites, Ampr | Morash et al. (2009) |
| pDK268 | pBH6119::PmagA 807-bp region cloned into BamHI and XbaI sites, Ampr | This study |
| pDK289 | pSK::PmagA 807-bp region and gfpmut3 cloned into BamHI and SacII sites, Ampr | This study |
| pDK298 | Downstream 817-bp region flanking magA cloned into SacII and SacI sites of pDK289, Ampr | This study |
| pKB398 | pSR47s:: PmagA 807-bp region, gfpmut3 and downstream flanking 817-BP region cloned into SalI and SacI sites; Kanr | This study |
| pKB9 | dotA expression plasmid; Ampr | R. Isberg (Roy et al. 1998) |
| pRL101 | sdhA expression plasmid; Ampr | R. Isberg (Laguna et al. 2006) |
| pL4440 | RNAi cloning vector for generating dsRNA; Ampr, Tetr | J. Kinchen (Timmons et al. 2001) |
| pMP371 | pL4440::ced-3 | This study |
| pBH6119 | Promoterless GFP vector; AmpR, Thy+ | M. Swanson (Hammer and Swanson 1999) |
| C. elegans | ||
| N2 | Wild-type isolate from Bristol, England | CGC (Brenner 1974) |
| GH403 | glo-3(kx94) | CGC (Rabbitts et al. 2008) |
| DH1201 | rme-1(b1045) | CGC (Grant et al. 2001) |
| MD701 | Plim-7ced-1::gfp | CGC (Schumacher et al. 2005) |