Table 2.
Oligonucleotides used in this study
| Purpose | Primers (5′→3′ direction)1 | Annealing temp. (°C) | Length (bp) |
|---|---|---|---|
| Amplicon | |||
| PmagA | PF GCGATAggatccGTAGCTGATTAATTGA | 51.5 | 807 |
| PR GCGATAtctagaGTAGCTGATTAATTGA | |||
| PmagA + gfpmut3 | PmagA PF | 57.5 | 1362 |
| PR GCGATAccgcggTTATTTGTACAATTCA | |||
| 3′ magA flank | PF GCGATAccgcggATTATTCTAAGGGGTG | 52.1 | 817 |
| PR GCGATAgagctcCGTCAGTATAGCCTT A | |||
| PmagA + gfpmut3 + 3′magA flank | PF GCGATAgtcgacGTAGCTGATTAATTGA | 57.5 | 2179 |
| 3′ magA flank PR | |||
| magA Int | PF GCACTGGAAGCCTACA | 57.5 | 273 |
| PR GATGTCGAACTCACTCA | |||
| ced-3 | PF CATggcgcgccATGAGCGATTACGACTTGAACTGTA | 51.5 | 595 |
| PR CATactagtCGTCAAGATAGAAGGAGCTTGCTA | |||