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. 2015 Jul 6;4(4):682–697. doi: 10.1002/mbo3.272

Figure 3.

Figure 3

Overexpression of yefM suppresses temperature-induced A-site mRNA cleavage. (A) flag-(m)ybeL-PP transcripts were expressed in ssrA− rnb+ and ssrA− Δrnb backgrounds at 37°C and 42°C. Where indicated (+), the yefM or relB antitoxin genes were overexpressed from a plasmid-borne arabinose-inducible promoter. (B) flag-(m)ybeL-PP transcripts were expressed in ssrA− rnb+ and ssrA− Δrnb backgrounds at 37°C. Where indicated (+), the yoeB or yefM-yoeB genes were overexpressed from plasmid-borne arabinose-inducible promoters. The migration positions of stop codon truncated messages are indicated by control transcripts prepared by in vitro transcription. The horizontal arrows indicate an additional truncated transcript that is produced during growth at 42°C (A) or yoeB induction without yefM (B).