Table 2.
Overview about previous metaproteome studies
| Author | Fermenter | Substrate | Process temperature | Separation method | Identified proteins |
|---|---|---|---|---|---|
| Abram et al. (2011) | 3–5 L lab scale | synthetic glucose-based wastewater | 15°C | 2D-PAGE (388 spots) | 33 proteins |
| Yan et al. (2012) | 2 L lab scale | blue algae, sludge | 35°C | 2D-PAGE (200–300 spots) | 3 proteins |
| Hanreich et al. (2012) | 8 L lab scale | beet silage, chopped rye | 55°C | 2D-PAGE (350 spots) | 7 enzymes of methanogenesis + several housekeeping proteins |
| Hanreich et al. (2013) | 500 ml batch test | straw, hay, digestate from maize fermentation | 38°C | 2D-PAGE (300 spots) | 80 proteins |
| Heyer et al. (2013) | 6 agricultural biogas plants | mainly grain silage, slurry and manure | 5× mesophilic 1× thermophilic | SDS-PAGE | 100–150 proteins |
| Kohrs et al. (2014) | mesophilic agricultural biogas plants | maize silage, forage rye, cattle manure and slurry | 43°C | LC-MS/MS, SDS-PAGE, Liquid-IEF + SDS-PAGE | 757–1,639 proteins |
| thermophilic agricultural biogas plants | maize whole crop silage and poultry manure | 52°C | LC-MS/MS, SDS-PAGE, Liquid-IEF + SDS-PAGE | 1,663–2,091 proteins | |
| Lü et al. (2014) | 1 L bottle | office paper + sludge + buffer | 55°C | LC-MS/MS, SDS-PAGE, Liquid-IEF | 500 proteins |