Table 1.

Summary of observed OTUs, Chao1, Shannon and Simpson index in manure (S1 and S2 correspond to duplicate samples) and in laboratory-scale digesters processing: (1) manure as sole substrate (RM); (2) stream-exploded straw and manure, operating constantly at 37°C (R^TcSS); and (3) steam-exploded straw and manure, operating temperatures 37°C, 44°C and 52°C (R³⁷SS, R⁴⁴SS and R⁵²SS), where R1 and R2 represent parallel digesters

Sample	Archaea				Bacteria
	Chao1	OTUs	Shannon	Simpson	Chao1	OTUs	Shannon	Simpson
Manure S1	22	20	2.081	0.648	358	270	6.361	0.971
Manure S2	27	25	2.404	0.693	420	274	6.167	0.957
RM Day 42	69	23	2.028	0.639	487	266	6.355	0.975
RM Day 85	18	13	1.848	0.607	553	277	6.292	0.972
RM Day 148	22	18	1.870	0.597	440	253	5.819	0.956
RTcSS R1 Day 9	26	18	2.369	0.747	320	191	4.896	0.910
RTcSS R2 Day 9	26	20	2.367	0.723	371	225	5.550	0.949
R37SS R1 Day 9	20	17	2.271	0.736	398	209	5.221	0.932
R37SS R2 Day 9	28	22	2.309	0.714	425	203	5.197	0.934
RTcSS R1 Day 38	23	20	2.643	0.777	309	197	5.463	0.952
RTcSS R2 Day 38	47	24	2.612	0.759	319	198	5.524	0.953
R37SS R1 Day 38	22	20	2.295	0.737	325	212	5.377	0.936
R37SS R2 Day 38	28	23	2.117	0.632	398	209	5.494	0.951
RTcSS R1 Day 80	55	20	2.390	0.744	421	244	6.134	0.969
RTcSS R2 Day 80	26	21	2.237	0.682	315	217	5.811	0.959
R37SS R1 Day 80	21	18	1.740	0.533	374	229	5.688	0.950
R37SS R2 Day 80	101	23	2.438	0.751	358	234	5.819	0.948
RTcSS R1 Day 99	21	18	2.000	0.639	375	235	5.988	0.965
RTcSS R2 Day 99	44	16	1.704	0.569	334	221	5.900	0.959
R37SS R1 Day 99	32	22	2.530	0.736	321	232	6.064	0.968
R37SS R2 Day 99	78	23	2.399	0.729	391	224	5.827	0.959
R44SS R1 Day 224	21	14	1.618	0.594	191	156	5.015	0.923
R44SS R2 Day 224	22	15	1.456	0.545	255	158	4.906	0.926
R52SS R1 Day 402	13	12	2.150	0.718	149	112	3.446	0.717
R52SS R2 Day 402	17	15	2.233	0.731	165	119	4.023	0.787