Skip to main content
. 2015 Sep 1;23(7):630–642. doi: 10.1089/ars.2014.6186

FIG. 4.

FIG. 4.

H2S lessens Dnmt3a expression. (A, B) Global DNA methylation is higher in CSE-deficient SMCs and aorta in mice. *p<0.05. (C) CSE deficiency induces the expression of Dnmt3a but not Dnmt1 and Dnmt3b in mouse aorta. *p<0.05. (D, E) Exogenously applied H2S reduces the mRNA and protein expression of Dnmt3a. WT-SMCs were incubated with NaHS (30 μM) for 48 h after detection of Dnmt3a mRNA and protein expression. *p<0.05. (F, G) CSE overexpression decreases Dnmt3a mRNA and protein expression. WT-SMCs were transfected with CSE cDNA for 48 h after detection of Dnmt3a mRNA and protein expression. *p<0.05. (H) H2S has no effect on the mRNA expression of Dnmt1, Dnmt3b, and Tet 1–3. WT-SMCs were incubated with NaHS (30 μM) for 48 h after detection of mRNA expression of Dnmt1, Dnmt3b, and Tet 1–3. All experiments were repeated at least thrice, and four to six mice were used in each group to isolate aorta tissue.