Figure 3.

rAAV2/6 transduction of neighboring tissues but not of the skin graft after intradermal vector injection. (a) In vivo imaging of luciferase expression after intradermal vector administration. Representative images show xenotransplanted mice (day 28) in groups injected intradermally with LV/CMV-FLuc (left) or rAAV2/6-CMV-FLuc (middle), as well as a saline-injected negative control mouse (right). (b) Ex vivo detection of luciferase activity in human skin xenografts exposed to LV/CMV-FLuc or rAAV2/6-CMV-FLuc and in a graft from a negative control mouse injected with saline. Shown are representative images of the human skin xenografts excised immediately after the mice were killed on day 28. LV, lentiviral vector; rAAV, recombinant adeno-associated viral vectors; p/sec/cm²/sr, photons per second per centimeter squared per steradian. Data are presented as means + SD.