Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Sep 1;23(7):597–612. doi: 10.1089/ars.2014.5939

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright 2015, Mary Ann Liebert, Inc.

PMC Copyright notice

FIG. 3. — Analysis of the protein folding, ER stress and degradation machineries in SC and OM adipocytes from NG and IR subjects. (A) HSP70 protein content in SC and OM adipocytes of NG and IR subjects (n=5 individuals/group). Standardized log. abundance of the spots in the 2D-DIGE gels (upper panels; n=4 individuals/group). (B) Protein content of the ER marker, CHOP, in adipocytes of NG and IR subjects (n=5 individuals/group). (C) Quantification of ubiquitinated proteins in NG and IR adipocytes (n=5 individuals/group); protein levels in SC-NG adipocytes in the dot blots were set to 1 and used as reference for data normalization. (D) Measurement of the chymotrypsin-like (ChT-L) peptidase activity of the 26S proteasome (+ATP) (left panel) and 20S proteasome (+SDS) (right panel) in SC and OM adipocytes of NG and IR subjects (n=5 individuals/group). Data are expressed as a percentage of values in SC-NG adipocytes (100%). *p<0.05 versus NG.