Table 2.

Proteins Identified by MALDI-MS/MS Differentially Expressed in Mature Subcutaneous and Omental Adipocytes from Normoglycemic and Insulin-Resistant Morbidly Obese Individuals

DIGE				Protein			Mascot
Spota	T-testb	Av ratioc	Accession numberd	Symbol	Name	Mw (kDa)/pIe	Pepf	% coverg	Expecth
Proteomic comparison of SC vs. OM adipocytes from NG subjects
1429	0.027	1.60	CAA00975	APOA1	Apolipoprotein A-I	30.7/5.5	21	54	2.0e-023
1505	0.015	1.43	NP_000468	ALBU	Serum albumin	71.3/5.9	15	21	1.0e-023
1572	0,025	1.97	Q13418	ILK	Integrin-linked protein kinase	51.8/8.3	9	16	4.0e-009
1543	0.040	−2.18	NP_000137	FRIL	Ferritin light chain	20.0/5.5	21	65	2.0e-067
726	0.018	−2.45	NP_000680	AL1A1	Retinal dehydrogenase 1	55.4/6.3	21	38	2.0e-037
735	0.029	−2.56	NP_000680	AL1A1	Retinal dehydrogenase 1	55.4/6.3	25	54	1.6e-045
953	0.026	−1.37	NP_001814	KCRB	Creatine kinase B-type	42.9/5.3	13	34	6.4e-010
1354	0.043	−1.48	NP_002620	PGAM1	Phosphoglycerate mutase 1	28.9/6.6	9	45	0.00018
1484	0.029	−1.42	NP_000467	KAD1	Adenylate kinase isoenzyme 1	21.7/8.7	18	65	8.1e-028
			NP_000468	ALBU	Serum albumin	71.3/5.9	9	15	1.3e-009
1459	0.025	−1.99	P63261	ACTG	Actin, cytoplasmic 2	42.1/5.3	11	34	2.0e-020
968	0.050	−1.50	NP_062800	IVD	Isovaleryl-CoA dehydrogenase, mitochondrial	46.8/8.4	16	34	3.1e-006
Proteomic comparison of SC vs. OM adipocytes from IR subjects
670	0.046	−1.72	NP_955472	CH60	60 kDa heat shock protein, mitochondrial	61.1/5.7	23	53	2.5e-049
675	0.049	−1.77	NP_000680	AL1A1	Retinal dehydrogenase 1	55.4/6.3	19	36	8.1e-029
801	0.007	−2.31	P21399	ACOC	Cytoplasmic aconitate hydratase	98.8/6.2	20	18	1.0e-026
Proteomic comparison of SC adipocytes of IR vs. NG subjects
826	0.038	1.32	NP_000169	GSHB	Glutathione synthetase	52.5/5.6	13	31	1.3e-009
1438	0.038	2.66	P28161	GSTM2	Glutathione S-transferase Mu 2	25.8/6.0	30	83	1.3e-048
1387	0.025	2.14	NP_006397	PRDX4	Peroxiredoxin-4	30.7/5.8	16	58	2.5e-031
924	0.039	1.91	Q99536	VAT1	Synaptic vesicle membrane protein VAT-1	42.1/5.8	13	40	9.0e-005
1484	0.003	2.81	NP_000467	KAD1	Adenylate kinase isoenzyme 1	21.7/8.7	18	65	8.1e-028
			NP_000468	ALBU	Serum albumin	71.3/5.9	9	15	1.3e-009
511	0.020	5.71	NP_000399	GPDM	Glycerol-3-phosphate dehydrogenase, mitochondrial	81.3/7.5	24	34	2.0e-014
827	0.010	2.34	NP_000009	ACADV	Very long-chain specific acyl-CoA dehydrogenase, mitochondrial	70.7/8.9	17	29	1.0e-028
983	0.041	23.42	NP_003371	VIME	Vimentin	53.6/5.0	31	56	2.0e-040
1314	0.044	3.25	CAA39992	CALB2	Calretinin	31.6/5.0	11	36	1.1e-005
1122	0.026	2.30	AAA18024	FIBB	Fibrinogen beta chain	56.5/8.5	27	43	1.6e-020
911	0.034	4.35	Q9P2R7	SUCB1	Succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial	50.6/7.0	18	29	5.1e-016
1441	0.050	3.30	Q9Y6M9	NDUB9	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9	22.0/8.5	10	48	4.0e-011
1455	0.043	−1.55	P48668	K2C6C	Keratin, type II cytoskeletal 6C	60.2/8.0	18	31	6.4e-023
			NP_009193	PARK7	Protein DJ-1/PARK7	20.0/6.3	9	60	0.0011
1241	0.024	−2.60	P15559	NQO1	NAD(P)H dehydrogenase [quinone] 1	30.9/8.9	16	44	3.2e-026
670	0.004	−2.46	NP_955472	CH60	60 kDa heat shock protein, mitochondrial	61.1/5.7	23	53	2.5e-049
1001	0.043	−1.88	NP_006588	HSP7C	Heat shock cognate 71 kDa protein	71.0/5.3	17	29	4.0e-025
			NP_005336	HSP71	Heat shock 70 kDa protein 1A/1B	70.2/5,4	14	23	3.2e-016
1038	0.036	−2.33	CAG46678	ALDOA	Fructose-bisphosphate aldolase A	39.8/8.3	23	67	1.0e-050
932	0.036	−1.66	P11310	ACADM	Medium-chain specific acyl-CoA dehydrogenase, mitochondrial	47.0/8.6	27	51	1.0e-046
939	0.016	−5.20	NP_001257	EST1	Liver carboxylesterase 1	62.7/6.1	20	41	5.1e-034
657	0.050	−3.59	AAB29178	PGM1	Phosphoglucomutase-1	61.6/6.3	20	37	2.0e-016
Proteomic comparison of OM adipocytes of IR vs. NG subjects
1572	0,025	1.9	Q13418	ILK	Integrin-linked protein kinase	51.8/8.3	9	16	4.0e-009
1044	0.019	1.99	CAA36267	CO6A3	Collagen alpha-3 (VI) chain	34.5/8.9	1	4	0.00068
711	0.046	3.39	NP_005304	PDIA3	Protein disulfide-isomerase A3	57.1/5.9	15	33	6.4e-013
1215	0.017	−1.58	NP_689953	3HIDH	3-hydroxyisobutyrate dehydrogenase, mitochondrial	35.7/8.3	14	33	5.1e-009
1496	0.014	−1.38	CAG28580	PRDX1	Peroxiredoxin-1	22.3/8.2	19	68	4.0e-046
			CAG46588	PRDX2	Peroxiredoxin-2	22.0/6.7	3	16	2.2e-006
1383	0.050	−1.66	NP_004896	PRDX6	Peroxiredoxin-6	25.1/6.0	26	83	1.6e-052

^aSpot numbers correspond to those in Figure 1A.

^bp-value of the Student's t-test. Only those proteins with a p≤0.05 were considered.

^cAverage volume ratio (^a,b,c were calculated by the DeCyder analysis).

^dAccession numbers from the NCBInr database.

^eTheoretical molecular weight (kDa) and isoelectric point (pI).

^fPep. corresponds to the number of peptides identified.

^gCoverage of all peptide sequences matched to the identified protein (%).

^hMascot expected value sequence (^f,g,h were provided by Mascot).