Table 3.

Effect of O. basilicum oil on the in vitro growth T. vaginalis trophozoites (10⁶) at different incubation periods

Dosage of treatment	After 24 h		After 48 h		After 72 h		After 96 h
	(Trophozoites number) Mean ± SD	% of growth inhibition	(Trophozoites number) Mean ± SD	% of growth inhibition	(Trophozoites number) Mean ± SD	% of growth inhibition	(Trophozoites number) Mean ± SD	% of growth inhibition
Parasite control	(2.10–2.36) 2.23 ± 0.13	0	(3.96–4.36) 4.16 ± 0.20	0	(11.28–11.52) 11.4 ± 0.12	0	(4.94–5.16) 5.05 ± 0.11	0
Metronidazole, 20 μg	(0.78–0.82) 0.8 ± 0.02*	64.1	(0.07–0.73) 0.4 ± 0.33*	90.4	(0.03–0.17) 0.1 ± 0.07*	99.1	0.00*	100
Metronidazole, 40 μg	0.00*	100	0.00*	100	0.00*	100	0.00*	100
Solvent control	(2.05–2.35) 2.2 ± 0.15	1.3	(4.00–4.22) 4.11 ± 0.11	1.2	(11.2–11.4) 11.3 ± 0.1	0.9	(4.91–5.09) 5.0 ± 0.09	1
Ocimum basilicum oil, 10 μg/ml	(1.6–1.8) 1.7 ± 0.1*,•	23.8	(0.88–0.92) 0.9 ± 0.02*,•	78.4	(0.45–0.55) 0.5 ± 0.05*,**	95.6	0.00*	100
Ocimum basilicum oil, 20 μg/ml	(0.5–0.7) 0.6 ± 0.1*,•	73.1	0.00*,•	100	0.00*,•	100	0.00*	100
Ocimum basilicum oil, 30 μg/ml	0.00*,**	100	0.00*,•	100	0.00*,•	100	0.00*	100

SD standard deviation

* p < 0.05, statistically significant difference in comparison to parasite control in the same time interval; ** p < 0.05, statistically significant difference in comparison to metronidazole 20 μg in the same time interval; ^• no statistically significant difference (p > 0.05) in comparison to metronidazole 20 μg