a) Effect of cysteine (1, 2, 5, 7 and 9 mM), (b) Ca2+ (0.1, 0.2, 0.3, 0.4 and 0.5 mM), (c) Cu2+ (0.1 mM, 0.3 mM, 0.5 mM, 0.7 mM, 0.9 mM), and (d) Hg2+ (0.3 mM, 0.6 mM, 1.0 mM, 1.3 mM, 1.6 mM) on the hydrolysis of gelain by bromelain extracted from Ananas comosus