Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Effect of Ca2+ on kinetic parameters of Bromelain at pH (3.5, 4.5, and 7.5)
| pH | Ca2+ = 0 mM | Ca2+ = 0.5 mM | ||
|---|---|---|---|---|
| Km (mM) | Vmax (GDU/g) | Km (mM) | Vmax (GDU/g) | |
| 3.5 | 15 ± 0.47 | 10000 ± 6.0 | 11.5 ± 0.05* | 5000 ± 5.03* |
| 4.5 | 28.6 ± 0.2 | 3333.3 ± 0.58 | 11 ± 0.100* | 5000 ± 1.15* |
| 7.5 | 29.4 ± 0.1 | 2000 ± 1.15 | 6.33 ± 0.01* | 3333.3 ± 0.75* |
Mean ± S.D (n = 3), p* < 0.001 vs control
