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Effect of Cu2+on kinetic parameters of Bromelain at pH (3.5, 4.5, and 7.5)
| pH | Cu2+ = 0 mM | Cu2+ = 0.6 mM | ||
|---|---|---|---|---|
| Km (mM) | Vmax (GDU/g) | Km (mM) | Vmax (GDU/g) | |
| 3.5 | 15 ± 0.47 | 10000 ± 6.0 | 35 ± 0.11* | 1000 ± 0.76* |
| 4.5 | 28.6 ± 0.2 | 3333.3 ± 0.58 | 11 ± 1.0* | 1000 ± 2.0* |
| 7.5 | 29.4 ± 0.1 | 2000 ± 1.15 | 15 ± 0.2* | 500 ± 1.39* |
Mean ± S.D (n = 3), p* < 0.001 vs control
