Fig. 1.

Genital mouse model of HPV-associated cancer. TC-1* cells were infected with Lenti-Luc vector to generate TC-1*-Luc cells. a Different concentrations of TC-1*-Luc cells (triplicates) were plated in 96-well plates, and D-luciferin (final concentration of 0.15 mg/ml) was added 15 min before monitoring. Bioluminescence was measured by imaging system and calculated as photons/s/cm²/sr. Each point is mean ± SD. b Diestrum synchronized eight-week-old female C57BL/6 mice were pre-treated with a spermicidal/detergent N9 (4 %), and after 6 h washed with phosphate-buffered saline before challenging with 5 × 10⁴ TC-1*-Luc cells. Genital tumour growth was monitored at the indicated time interval post-challenge. Bioluminescence was measured 15 min after intraperitoneal injection of D-luciferin by imaging system and quantified as photons/s/cm²/sr in a colour scale