Heart homogenates from vehicle-, SFN-, DOX- and SFN+DOX-treated mice were examined by western blot analysis for levels of NQO-1, HO-1, SOD2, GSTA4-4 and β-actin (n=4–6 per group). Grayscale values of the GSTA4-4, SOD2, NQO-1 and HO-1 bands, normalized with β-actin using ImageJ software (developed by NIH) are shown under the corresponding bands to indicate the relative expression level of proteins in a representative western blot.