Table 2. Relative levels of Nrf2 target gene transcripts from control and treated mice.

	CONTROL	SFN	DOX	DOX+SFN
Cat	0.92±0.10	2.71±0.41*	0.28±0.03*	0.82±0.06
Sod1	3.96±0.26	7.49±0.53*	1.08±0.36*	3.22±0.31
Sod2	3.58±0.17	12.40±0.83*	1.31 ±0.17*	4.39±0.12
Pxdn	0.32±0.08	0.31 ±0.16	0.33±0.09	0.29±0.14
Gpx1	1.00±0.07	1.77±0.18*	0.41±0.15*	0.83±0.53
Gpx4	2.43±0.54	2.88±0.86	2.22±0.39	1.91 ±0.49
Txnrd1	0.02±0.01	0.02±0.01	0.03±0.01	0.03±0.01
HO-1	0.13±0.06	0.36±0.05*	0.06±0.03*	0.18±0.02
NQO1	0.36±0.03	1.07±0.20*	0.17±0.05*	0.30±0.03
Gsta1+2	0.03±0.01	0.05±0.02	0.05±0.01	0.02±0.01
Gsta3	0.07±0.02	0.08±0.03	0.06±0.01	0.06±0.05
Gsta4	1.90±0.81	4.53±1.18*	1.08±0.14*	2.09±0.48
Gstm1	0.65±0.24	0.76±0.30	1.18±0.31*	0.83±0.25
Aor	0.02±0.01	0.01±0.01	0.03±0.01	0.01±0.001
Akr3	2.47±0.16	1.62±0.42	0.59±0.06*	0.89±0.16*
Akr8	0.38±0.11	0.13±0.08*	0.10±0.05*	0.23±0.19
Gclc	0.13±0.02	0.17±0.01 *	0.06±0.02*	0.10±0.02
Gclm	0.06±0.01	0.05±0.01	0.04±0.02	0.05±0.03

Levels of Nrf2-regulated transcripts encoding antioxidant and anti-electrophile enzymes, and enzymes involved in glutathione biosynthesis were measured by qRT-PCR in hearts of control and treated animals. Gene expression levels were normalized to the S3 ribosomal protein transcript and calculated as described in the Materials and methods. Asterisks denote statistically significant differences (p < 0.05; n = 5) between control and treated groups of animals, compared pairwise.