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. Author manuscript; available in PMC: 2016 Sep 11.
Published in final edited form as: Biochem Biophys Res Commun. 2015 Aug 1;465(1):152–158. doi: 10.1016/j.bbrc.2015.07.156

Fig. 4. Analysis of corin mutants with full-length cytoplasmic tails lacking the KFQ motif.

Fig. 4

(A) Illustration of corin mutants with full-length cytoplasmic tails. Residues 7–59 are not shown. The KFQ motif is boxed. (B, C) Western analysis of corin mutants in transfected HEK293 cells under reducing conditions. Percentage of corin activation (Corin-p vs. Corin bands) were calculated based on densitometry of Western blots. n=3 per group. (D, E) Flow cytometric analysis of surface corin in HEK293 cells transfected with mWT and mK71A/F72A/Q73A plasmids. Data are mean ± S.D. of corin-positive cells. n=5 per group.