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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Free Radic Biol Med. 2015 Jun 6;86:250–258. doi: 10.1016/j.freeradbiomed.2015.05.032

Fig. 3.

Fig. 3

Mitochondrial matrix accumulation is required for MitoSOX inhibition of uncoupled respiration. In A, primary rat cortical neurons were treated with FCCP plus pyruvate (FCCP+pyr, 5 μM and 10 mM, respectively, first arrow), followed by vehicle control (con, filled circles) or MitoSOX (10 μM, open circles), and finally with a combination of rotenone (rot, 1 μM) and antimycin A (AA, 1 μM). In B, neurons in other wells on the same plate were first treated with MitoSOX or vehicle control, then with FCCP+pyr and rot+AA. Results in A and B are mean ± SD (n=3 wells) and are representative of two independent experiments. OCR is baseline-normalized to the point immediately prior to the first addition.