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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Free Radic Biol Med. 2015 Jun 6;86:250–258. doi: 10.1016/j.freeradbiomed.2015.05.032

Fig. 8.

Fig. 8

Low micromolar MitoSOX causes mitochondrial bioenergetic dysfunction in microglial cells. Rat HAPI (A) or mouse BV2 (B) microglial cells were treated with vehicle control (con, filled circles) or MitoSOX at the indicated concentrations after three baseline O2 consumption rate (OCR) measurements (first arrow). Subsequently, oligomycin (oligo, 0.3 μg/ml), FCCP plus pyruvate (FCCP+pyr, 5 μM and 10 mM, respectively), and a combination of rotenone (rot, 1 μM) and antimycin A (AA, 1 μM) were added as indicated (arrows). Results in A and B are mean ± SD (n=4 wells) and are representative of two independent experiments. OCR is baseline-normalized to the point immediately prior to MitoSOX addition. Numbers in legends correspond to MitoSOX concentration in μM.