Table 3.

Effects of nitrate on common classified bacteria genera in ruminal fluid ranked by average relative abundance (% of total sequences) using NGS technology

Phyla	Taxon	Treatments			SEM	p-value	Contrast
	Genus	0NR	1NR	2NR			PL	PQ
Bacteroidetes	Prevotella	15.56	17.04	15.80	2.09	0.87	0.94	0.62
Bacteroidetes	BF311	2.60	4.74	2.86	0.53	0.06	0.74	0.02
Bacteroidetes	CF231	2.17	2.61	2.24	0.12	0.08	0.71	0.03
Bacteroidetes	YRC22	1.77a	0.70b	0.89b	0.23	0.03	0.03	0.07
Bacteroidetes	Paludibacter	0.16	0.11	0.06	0.04	0.34	0.16	0.88
Fibrobacteres	Fibrobacter	0.71	1.06	0.83	0.20	0.49	0.70	0.27
Firmicutes	Ruminococcus	2.28	1.44	1.18	0.37	0.17	0.08	0.54
Firmicutes	Succiniclasticum	1.41	1.10	1.59	0.23	0.38	0.60	0.21
Firmicutes	Clostridium	1.03	1.10	1.26	0.15	0.57	0.32	0.82
Firmicutes	Butyrivibrio	0.75	0.93	0.80	0.05	0.13	0.56	0.06
Firmicutes	Coprococcus	0.41	0.36	0.54	0.08	0.36	0.32	0.30
Firmicutes	RFN20	0.28	0.42	0.42	0.07	0.32	0.21	0.41
Firmicutes	Oscillospira	0.31	0.31	0.45	0.07	0.37	0.23	0.47
Proteobacteria	Campylobacter	0.04b	0.48b	3.49a	0.43	<0.01	<0.01	0.05
Proteobacteria	Ruminobacter	0.53ab	0.95a	0.15b	0.15	0.03	0.12	0.02
Proteobacteria	Succinivibrio	0.28	0.64	0.18	0.14	0.13	0.63	0.06
Proteobacteria	Acinetobacter	0.16	0.07	0.30	0.08	0.18	0.25	0.13
Proteobacteria	Lampropedia	0.04	0.04	0.32	0.11	0.18	0.11	0.32
Proteobacteria	Desulfovibrio	0.10	0.12	0.15	0.03	0.45	0.23	0.93
Proteobacteria	Pseudomonas	0.12	0.11	0.08	0.04	0.79	0.52	0.90
Spirochaetes	Sphaerochaeta	0.15b	0.40a	0.08b	0.05	0.01	0.36	<0.01
Spirochaetes	Treponema	0.14	0.15	0.23	0.04	0.32	0.17	0.55

NGS, next-generation DNA sequencing; 0NR, control; 1NR, 1% nitrate; 2NR, 2% nitrate; SEM, standard error of the mean.

¹P_L is liner tendency; P_Q is quadratic tendency.

Different superscripts in the same row represent significant differences (p<0.05).