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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: J Virol Methods. 2015 Jul 20;223:25–29. doi: 10.1016/j.jviromet.2015.07.006

Fig. 4.

Fig. 4

Intracellular staining (ICS) of JC virus VP1 protein in 293FT cells transfected or infected with JCV Mad1 (A,B) or JCV iLOV-T (C,D). JCV VP1 ICS is performed as described previously, using fluorescent reporter Alexa Fluor 568. 293FT cells 18 days post transfection (upper panels) or 7 days post infection (lower panels) are shown. Cells that are red express JCV VP1 protein