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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2015 Jul 26;1848(10 0 0):2374–2384. doi: 10.1016/j.bbamem.2015.07.013

Fig 1.

Fig 1

Fluorescently-labeled ceramide binds to Bcl-xL. A. Separation of fluorescently-labeled ceramide bound Bcl-xL from ceramide micelles. F1 is fluorescently-labeled ceramide: C11 TopFluor ceramide. B. Fluorescence spectrum of final sample from the separation shown in “A” (see methods for details). “WT” indicates the medium contained wild-type Bcl-xL and fluorescence was due to bound fluorescently-labeled ceramide. “buffer” indicates that there was no Bcl-xL in that separation procedure. The lack of fluorescence indicates that the procedure was effective at eliminating ceramide micelles. The inset is the structure of fluorescently-labeled ceramide.