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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Free Radic Biol Med. 2015 Apr 25;86:25–36. doi: 10.1016/j.freeradbiomed.2015.04.009

Fig. 1. Analysis of σ1R expression in mouse Müller cells.

Fig. 1

(A) Müller cells harvested from WT (wildtype) and σ1RKO (knockout) mice were cultured as described and subjected to immunocytochemistry to confirm the glial origin of the cells using vimentin (green fluorescence) or to detect σ1R (red fluorescence); DAPI was used to label nuclei blue. Calibration bar = 100 μm. (B) Immunoblotting analysis to detect σ1R in WT and σ1RKO Müller cells, the single band detected in WT samples has the expected size Mr 25–27 kD. Membranes were reprobed with antibody against GAPDH as a loading control.