Figure 4.

The role of ERK, Rho, GSK and Dex in the expression of epithelial and mesenchymal markers in psoriatic lesional keratinocytes. (a,b) Psoriatic keratinocytes were treated with U0126, and indicated target gene was determined by (a) Western blot (one representative example out of at least three similar repeats) and (b) qRT-PCR separately. (c–f) Psoriatic keratinocytes were pretreated with Y27632 or SB216763 for 2 h, then incubated with or without Dex at 0.2 or 2.0 µM. Indicated target gene was determined by Western blot and qRT-PCR separately. β-actin is used as loading control in (a) and (c). U0126, ERK inhibitor; Y27632, Rho inhibitor; SB216763, GSK3 inhibitor.