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. 2015 Aug 19;5(8):150067. doi: 10.1098/rsob.150067

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© 2015 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 3. — The ER is organized by centrosomes during embryonic nuclear divisions. (a) A stage 9 embryo expressing GFP-Sec61α was imaged live through the course of a single mitotic division. The location of the two centrosomes (arrows) is clear in the metaphase image; note the radial organization of the ER around the centrosomes. (b) A Rtnl1-GFP (green) expressing embryo was injected with rhodamine-labelled α-tubulin (red). Fallout of a single nucleus (pink asterisk) can be seen between the 0:00 and 10:50 timepoints. As the syncytium begins the next mitosis (15:50 timepoint), two free centrosomes that organize ER membranes can be detected (arrows). These free centrosomes maintain ER association during metaphase (18:00 timepoint), when other centrosomes still associated with nuclei have formed spindles (arrowheads). Times, min:s; scale bar, 5 µm.