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. 2015 Aug 19;5(8):150067. doi: 10.1098/rsob.150067

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© 2015 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 5. — The ER envelope associates with peripheral interpolar spindle MTs. (a) A fixed GFP-Sec61α (green) spermatocyte at metaphase of the first meiotic division was immunostained for α-tubulin (red), asterless (Asl, blue) to localize centrosomes and phosphorylated histone 3 (pH3, blue). The ER envelope (arrowheads) overlaps with the outermost interpolar MTs of the spindle. (b) A metaphase spermatocyte fixed and stained as in (a) was imaged perpendicular to the spindle axis. Clear deformations of the ER envelope (arrowheads) are associated with interpolar bundles of MTs. (c) A meiotic GFP-Sec61α expressing spermatocyte that divided with its spindle perpendicular to the imaging plane was imaged live. Inward deformations of the ER envelope are also seen here (arrowheads). (d) A mitotic GFP-Sec61α expressing NB was imaged live perpendicular to the spindle axis to show deformations of the ER envelope (arrowheads). Times, min:s; scale bar, 5 µm.