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Eras expression in embryos was inhibited by CHIR99021 treatment. (a) In situ hybridization showed that Eras mRNA was decreased in E6.5 embryos treated with CHIR99021 (30 µM) for 15 h. Scale bar, 100 µm. (b) Eras mRNA was detected with real-time RT-PCR in E6.5 embryos treated with CHIR99021 (30 µM) for 15 h. (c) CHIR99021 (3 µM) treatment for 24 h decreased Eras expression in blastocysts cultured in vitro. (Student's t-test; **p < 0.01).
