Figure 4.

CRISPR/Cas9-mediated genome editing in hair cells in vivo. Cas9 protein and the GFP gRNA were complexed with the commercial lipids (Lipofectamine 2000), which were then injected into postnatal Atoh1-GFP mouse cochlea in which all outer hair cells were GFP positive. Genome editing was achieved in the outer hair cells by the absence of GFP signal in the injected inner ear, without affecting our hair cell survival. High-throughput sequencing confirmed the disruption of the GFP gene by indels. OHC: Outer Hair Cells