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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Free Radic Biol Med. 2015 May 28;86:308–321. doi: 10.1016/j.freeradbiomed.2015.05.018

Figure 7. Evaluation of redox-active drugs in an E. coli model of H2O2-induced damage - drugs were added to E coli–containing medium in parallel with H2O2.

Figure 7

20 µM compounds were exposed to H2O2 for 15 min. The parental (MG1655) strain (A) and catalase/peroxidase-deficient mutant (LC106) (B) were studied; with parental MG1655 5 mM H2O2, and with catalase/peroxidase LC106 0.5 mM H2O2 was used. The viability was measured via MTT test and expressed as a percentage of the MTT reduction by non-treated cells. At 5 mM H2O2, no compound was efficacious in protecting parental strain. At 0.5 mM H2O2, the Mn complexes that are relatively stable, or have modest kcat(H2O2), that results in relatively high O2 production yield, were able to dismute and remove H2O2, protecting cell against it. Such interaction, in the case of Fe porphyrin, eliminated both the toxicity of FeP and H2O2. Student t-test was used to determine the statistical significance. Mean ± S.E is presented. # statistical significance (p<0.05) compared to untreated cells; $ statistical significance (p<0.05) compared to cells treated with H2O2 only.