Table 1.
Information of primers used to evaluate Schwann cell enrichment, demonstration of hSOD1G93A in sciatic nerve and Schwann cell samples by PCR and to SYBR qPCR experiments in the sciatic nerve and Schwann cells isolated by means of flow cytometry sorting of 60-day-old pre-symptomatic SOD1G93A mice.
| Gene ID | Primer sequences (5′–3′) | Amplicon (BP) | |
|---|---|---|---|
| S100 | F: CCCTCATTGATGTCTTCCACC | R: TCTCCATCACTTTGTCCACC | 150 |
| Thy1 | F: GTCCTTACCCTAGCCAACTTC | R: CCGCCACACTTGACCAG | 134 |
| hSOD1G93A | F: ATCAGCCCTAATCCATCTGA | R: CGCGACTAACAATCAAAGTGA | 236 |
| Ngfr | F: CCTCATTCCTGTCTATTGCTCC | R: TGGCTCCTTGTTTATTTTGCTTG | 107 |
| H2afx | F: TTGTTCGCAGCTCTTCTACC | R: GTAGTGGCCTTTCCGCAG | 149 |
| Mapk10 | F: TGTTAGTGATTGACCCAGCG | R: TGTGCTCCCTTTCATCCAG | 141 |
| Cdkn1a | F: CAGATCCACAGCGATATCCAG | R: AGAGACAACGGCACACTTTG | 103 |
| Cdkn1b | F: TGGACCAAATGCCTGACTC | R: GGGAACCGTCTGAAACATTTTC | 144 |
| E2f1 | F: TCTCTTTGACTGTGACTTTGGG | R: TCGTGCTATTCCAATGAGGC | 147 |
| Traf2 | F: ACTTCACCAGAAAGCGTCAG | R: GGTTTTCTCTGTAGGTCTTCCG | 148 |
| Foxo3 | F: CGTTGTTGGTTTGAATGTGGG | R: TGCATCACTCGTTCATCCTG | 143 |
| Mtor | F: ATTCAATCCATAGCCCCGTC | R: ACAGTTCCAAAGACACCAGAG | 143 |
| Erbb3 | F: GGGCTATGAGACGCTACTTG | R: TGCAGGACAAACTAAGGAGTG | 145 |
| Hspa1a | F: TCGAGGAGGTGGATTAGAGG | R: TGCAGGACAAACTAAGGAGTG | 120 |
| Prdx2 | F: CCCTGAATATCCCTCTGCTTG | R: TTGACTGTGATCTGGCGAAG | 139 |
| NORMALYZER | |||
| Actb | F: ACCTTCTACAATGAGCTGCG | R: CTGGATGGCTACGTACATGG | 147 |
S100 (S100 calcium binding protein), Thy1 (THYmocyte differentiation antigen 1), hSOD1G93A (mutated human superoxide dismutase 1), Ngfr (Nerve growth factor receptor), H2afx (H2A histone family, member X), Mapk10 (mitogen-activated protein kinase 10), Cdkn1a (cyclin-dependent kinase inhibitor 1A (P21)), Cdkn1b (cyclin-dependent kinase inhibitor 1B), E2f1 (E2F transcription factor 1), Traf2 (TNF receptor-associated factor 2), Foxo3 (forkhead box O3), Mtor (mechanistic target of rapamycin-serine/threonine kinase), Erbb3 (v-erb-b2 erythroblastic leukemia viral oncogene homolog 3-avian), Hspa1a (heat shock protein 1A), Prdx2 (peroxiredoxin 2) and Actb (Actin, Beta).