Table 1.

Peptide selection: starting from 22 validated peptides of CSF tau protein obtained with high resolution mass spectrometry in PRM mode (Barthelemy, under review), the 7 quantifed peptides (GAAPPGQK, SGYSSPGSPGTPGSR, TPSLPTPPTREPK, TPSLPTPPTR, LQTPVPMPDLK, IGSTENLK, SPVVSGDTSPR) were selected by taking into account the three following parameters: signal intensities, presence of interferences, and concentration in CSF pools.

Peptide sequence	Peak intensity	Interfered	Increasing concentration in CSF pools	MRM validation
GAAPPGQK	600	No	Yes	Yes
TPPAPK	2200	No	No	No
TPPSSGEPPK	200	Yes	No	No
IGSTENLK	100	No	Yes	Yes
SPVVSGDTSPR	100	No	Yes	Yes
SGYSSPGSPGTPGSR	300	No	Yes	Yes
VQIINK	400	Yes	No	No
SRTPSLPTPPTREPK	0	Yes	No	No
IGSLDNITHVPGGGNK	0	Yes	No	No
ESPLQTPTEDGSEEPGSETSDAK	0	Yes	No	No
TPSLPTPPTREPK	1200	No	Yes	Yes
STPTAEDVTAPLVDEGAPGK	0	Yes	No	No
TPSLPTPPTR	400	No	Yes	Yes
QAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQAR	0	Yes	No	No
HVPGGGSVQIVYKPVDLSK	0	Yes	No	No
TDHGAEIVYK	0	Yes	No	No
DQGGYTMHQDQEGDTDAGLK	0	Yes	No	No
LDLSNVQSK	0	Yes	No	No
QEFEVMEDHAGTYGLGDR	0	Yes	No	No
LQTAPVPMPDLK	100	No	Yes	Yes
HLSNVSSTGSIDM(diox)VDSPQLATLADEVSASLAK	0	Yes	No	No
KLDLSNVQSK	0	Yes	No	No

Bold and italics correspond to the 7 chosen tau peptides.

Underline characters (M) correspond to oxidized methionine.