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. 2015 Sep 1;6:895. doi: 10.3389/fmicb.2015.00895

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Copyright © 2015 Thanapati, Das and Tripathy.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IFN-γ responses by ELISPOT. Box plots are showing IFN-γ responses by PBMCs from 18 control subjects, 13 acute, and 15 convalescent patients, stimulated with CHIK antigen (inactivated CHIKV 10 μg/10⁶ PBMCs). Y axis is showing spot forming cells (SFCs) per 10⁵ million cells. Plot shows IFN-γ responses by unstimulated PBMCs before subtraction from stimulated PBMCs well (spontaneous IFN-γ production) and after subtraction of spots in unstimulated PBMCs well from stimulated well (CHIKV specific IFN-γ production). Non-parametric Kolmogorov–Smirnov test was used for intergroup comparison. p < 0.05 is considered significant.