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. 2015 Sep 1;5:13558. doi: 10.1038/srep13558

Figure 6. Nf-κb and STAT3 inhibitors attenuated cytokine- and LPS-induced elevations in MDR1 gene expression in human T-lymphocytes and monocytes in vitro.

Figure 6

CCRF-CEM cells were cultured with 50 ng/ml TNF-α (A), 200 ng/ml IL-17 (B), 50 ng/ml IL-6 (C), 50 ng/ml IL-1β (D) and 2 μg/ml LPS (E) in the absence or presence of the Nf-κb inhibitor Bay 117082 (2 μM) and the STAT3 inhibitor Stattic (2 μM). THP-1 cells were also incubated with 50 ng/ml TNF-α (F), 200 ng/ml IL-17 (G), 50 ng/ml IL-6 (H), 50 ng/ml IL-1β (I) and 2 μg/ml LPS (J) with or without the Nf-κb inhibitor Bay 117082 (2 μM) and the STAT3 inhibitor Stattic (2 μM). The mRNA levels of MDR1 in the CCRF-CEM and THP-1 cells were measured by qPCR. Actin was used as an internal control. All of the data are shown as the mean ± S.E.M.; *p < 0.05, **p < 0.01 compared with the corresponding control group; n = 3.