Table 1. Summary for Musca domestica combined transcriptome.
| Parameters | Output of RNA-seq | Parameters | Assembly Quality |
|---|---|---|---|
| Total no. of raw reads | 63,490,654 | Total no. of contigs | 223,936 |
| Total no. of clean reads | 54,821,138 | Mean length of contigs (nt) | 258 |
| Mean length of clean reads (nt) | 90 | N50 of contigs (nt) | 326 |
| Total clean nucleotidesa (nt) | 4,933,902,420 | Total no. of unigenes | 89,842 |
| Q20 percentage | 96.25% | Mean length of unigenes (nt) | 532 |
| N percentage | 0.00% | N50 of unigenes (nt) | 665 |
| GC percentage | 53.69% | Distinct clusters | 30,105 |
| Distinct singletons | 59,737 |
a
Total clean nucleotides = total clean reads 1 × reads 1 size + total clean reads 2 × reads 2 size.