Table 6. Putative genes identified in connection with proPO system in M. domestica transcriptome.
| Unigene ID | Nucleotide Size (nt) | Predicted Protein Domainsa | Signal Peptideb | Hit-ACC [Species] | E-value | Annotated Function | Putative Genec |
|---|---|---|---|---|---|---|---|
| Peptidoglycan recognition receptor of M. domestica (MdPGRP) | |||||||
| Unigene46163 | 687 | PGRP(27-169); Aim_2(40-175) | 1-23 | Q9VYX7 [Drosophila melanogaster] | 8e-64 | PGRP SA | MdPGRP SA |
| Unigene46164 | 685 | PGRP(35-177); Aim_2(48-183) | 1-24 | Q9VYX7 [Drosophila melanogaster] | 2e-68 | PGRP SA | MdPGRP SA |
| Unigene653 | 682 | PGRP(35-177); Aim_2(48-183) | 1-24 | Q9VYX7 [Drosophila melanogaster] | 2e-69 | PGRP SA | MdPGRP SA |
| CL5483.Contig1 | 719 | PGRP(22-165); Aim_2(33-171) | 1-21 | Q70PY2 [Drosophila melanogaster] | 8e-78 | PGRP SB1 | MdPGRP SB |
| CL5483.Contig2 | 672 | PGRP(21-164); Aim_2(32-170) | 1-20 | Q70PY2 [Drosophila melanogaster] | 1e-79 | PGRP SB1 | MdPGRP SB |
| CL5483.Contig3 | 696 | PGRP(21-164); Aim_2(32-170) | 1-20 | Q70PY2 [Drosophila melanogaster] | 4e-79 | PGRP SB1 | MdPGRP SB |
| Unigene41919 | 646 | PGRP(20-162); Aim_2(33-168) | 1-19 | ADI87391.1 [Lucilia sericata] | 2e-71 | PGRP | MdPGRP SC |
| CL9661.Contig2 | 593 | PGRP(22-164); Aim_2(31-170) | 1-21 | ADI87391.1 [Lucilia sericata] | 1e-78 | PGRP | MdPGRP SC |
| CL4993.Contig1 | 593 | PGRP(22-164); Aim_2(33-170) | 1-21 | ADI87391.1 [Lucilia sericata] | 1e-89 | PGRP | MdPGRP SC |
| CL4993.Contig2 | 664 | PGRP(22-164); Aim_2(33-170) | 1-21 | ADI87391.1 [Lucilia sericata] | 1e-94 | PGRP | MdPGRP SC |
| Unigene25719 | 829 | PGRP(27-170); Aim_2(44-176) | 1-25 | XP_002094076.1 [Drosophila yakuba] | 4e-46 | PGRP SD | MdPGRP SD |
| Unigene34801 | 718 | PGRP(24-167); Aim_2(39-173) | 1-22 | Q70PR8.1 [Drosophila simulans] | 5e-45 | PGRP SD | MdPGRP SD |
| CL175.Contig1 | 536 | PGRP(24-157); Aim_2(42-157) | 1-22 | Q70PR8.1 [Drosophila simulans] | 9e-30 | PGRP SD | MdPGRP SD |
| CL3331.Contig2 | 776 | PGRP(102-244); Aim_2(116-250) | — | XP_002084233.1 [Drosophila simulans] | 1e-89 | PGRP LA | MdPGRP LA |
| CL5285.Contig1 | 801 | PGRP(22-165); Aim_2(33-171) | 1-17 | DQ307160.1 [Glossina morsitans morsitans] | 2e-14 | PGRP LB | MdPGRP LB |
| CL5285.Contig2 | 784 | PGRP(23-166); Aim_2(34-172) | — | DQ307160.1 [Glossina morsitans morsitans] | 2e-14 | PGRP LB | MdPGRP LB |
| Unigene47733 | 1076 | PGRP(166-309); Aim_2(180-315) | — | DQ307161.1 [Glossina morsitans morsitans] | 2e-08 | PGRP LC | MdPGRP LC |
| CL7750.Contig1 | 834 | PGRP(177-254) | — | NP_648298.1 [Drosophila melanogaster] | 2e-49 | PGRP LCb | MdPGRP LC |
| CL7750.Contig2 | 771 | PGRP(156-233) | — | ABC25065.1 [Glossina morsitans morsitans] | 1e-41 | PGRP LC | MdPGRP LC |
| Unigene46613 | 1366 | PGRP(166-309); Aim_2(178-315) | — | Q9VXN9 [Drosophila melanogaster] | 6e-69 | PGRP LE | MdPGRP LE |
| Unigene35645 | 601d | PGRP(1-133), Aim_2(2-139) | — | Q9VXN9 [Drosophila melanogaster] | 1e-44 | PGRP LE | MdPGRP LE |
| Prophenoloxidase of M. domestica (MdproPO) | |||||||
| CL572.Contig2 | 759 | Hemocyanin_M(2-140); Tyrosinase(8-144); Hemocyanin_C(146-229) | — | AAR84669.1 [Musca domestica] | 5e-123 | proPO | MdproPO 1 |
| CL572.Contig3 | 1291 | Hemocyanin_M(3-140); Tyrosinase(9-144); Hemocyanin_C(146-402) | — | AAR84669.1 [Musca domestica] | 0.0 | proPO | MdproPO 1 |
| CL572.Contig4 | 2255 | Hemocyanin_N(2-122); Hemocyanin_M(101-395); Tyrosinase(177-399); Hemocyanin_C(401-657) | — | AF161260.1 [Neobellieria bullata] | 0.0 | proPO 1 | MdproPO 1 |
| CL572.Contig5 | 1628 | Hemocyanin_M(1-213); Tyrosinase(4-217); Hemocyanin_C(219-475) | — | AF161260.1 [Neobellieria bullata] | 0.0 | proPO 1 | MdproPO 1 |
| CL572.Contig6 | 2215 | Hemocyanin_N(4-142); Hemocyanin_M(120-415); Tyrosinase(197-419); Hemocyanin_C(421-677) | — | AAR84669.1 [Musca domestica] | 0.0 | proPO | MdproPO 1 |
| Unigene49966 | 1668 | Hemocyanin_M(1-213); Tyrosinase(4-217); Hemocyanin_C(219-475) | — | AF161260.1 [Neobellieria bullata] | 0.0 | proPO 1 | MdproPO 1 |
| Unigene33273 | 2190 | Hemocyanin_N(24-145); Hemocyanin_M(149-416); Tyrosinase(197-420); Hemocyanin_C(422-682) | — | AF161261.1 [Neobellieria bullata] | 0.0 | proPO 2 | MdproPO 2 |
| Unigene9393 | 1330 | Hemocyanin_M(2-191); Tyrosinase(53-195); Hemocyanin_C(197-420) | — | AF161261.1 [Neobellieria bullata] | 0.0 | proPO 2 | MdproPO 2 |
| Prophenoloxidase activating factor of M. domestica (MdPAP) | |||||||
| Unigene46765 | 1297 | CLIP(42-95);Tryp_SPc(132-391) | 1-25 | P13582 [Drosophila melanogaster] | 3e-148 | Serine protease easter | MdPAP 1 |
| CL4801.Contig2 | 1334 | CLIP(30-83);Tryp_SPc(108-367) | 1-24 | P13582 [Drosophila melanogaster] | 4e-104 | Serine protease easter | MdPAP 1 |
| CL1201.Contig1 | 1491 | CLIP(51-104);Tryp_SPc(142-381) | 1-34 | P13582 [Drosophila melanogaster] | 1e-150 | Serine protease easter | MdPAP 1 |
| CL1201.Contig2 | 1190 | CLIP(42-95);Tryp_SPc(132-364) | 1-25 | P13582 [Drosophila melanogaster] | 2e-152 | Serine protease easter | MdPAP 1 |
| CL2563.Contig1 | 1271 | CLIP(29-88);Tryp_SPc(120-380) | 1-21 | P13582 [Drosophila melanogaster] | 5e-105 | Serine protease easter | MdPAP 1 |
| CL9802.Contig1 | 1479 | CLIP(39-91);Tryp_SPc(118-368) | 1-35 | P13582 [Drosophila melanogaster] | 7e-70 | Serine protease easter | MdPAP 2 |
| CL9736.Contig2 | 1255 | CLIP(39-93);Tryp_SPc(142-391) | 1-35 | P13582 [Drosophila melanogaster] | 2e-69 | Serine protease easter | MdPAP 2 |
| CL4876.Contig1 | 965 | Tryp_SPc(52-278) | 1-27 | ACC93936.1 [Musca domestica] | 3e-94 | Prophenoloxidase activating factor | MdPAP 3 |
| CL4876.Contig2 | 974 | Tryp_SPc(53-279) | 1-29 | ACC93936.1 [Musca domestica] | 1e-93 | Prophenoloxidase activating factor | MdPAP 3 |
| CL4876.Contig3 | 1006 | Tryp_SPc(53-283) | 1-29 | ACC93936.1 [Musca domestica] | 2e-68 | Prophenoloxidase activating factor | MdPAP 3 |
| CL14909.Contig1 | 1730 | CLIP(36-92,115-168);1Tryp_SPc(204-447) | 1-23 | P21902 [Tachypleus tridentatus] | 2e-59 | Proclotting enzyme | MdPAP 3 |
| CL16739.Contig2 | 1584 | CLIP(3-59,141-194);Tryp_SPc(232-476) | — | P21902 [Tachypleus tridentatus] | 9e-58 | Proclotting enzyme | MdPAP 3 |
| Serine protease inhibitors of M. domestica (MdSerpin) | |||||||
| Unigene46543 | 1509 | Serpin(40-403) | 1-24 | ABC25072.1 [Glossina morsitans morsitans] | 3e-124 | Serine protease inhibitor 1 | MdSerpin 1 |
| CL1146.Contig4 | 1298 | Serpin(38-402) | 1-17 | ABC25072.1 [Glossina morsitans morsitans] | 2e-123 | Serine protease inhibitor 1 | MdSerpin 1 |
| Unigene48882 | 1277 | Serpin(41-395) | 1-27 | XP_005177806.1 [Musca domestica] | 0.0 | Antitrypsin-like | MdSerpin 2 |
| CL5316.Contig7 | 1383 | Serpin(53-410) | 1-28 | XP_005177808.1 [Musca domestica] | 4e-162 | Serine protease inhibitor 3/4-like | MdSerpin 3 |
| CL8948.Contig2 | 1421 | Serpin(65-420) | — | XP_005177808.1 [Musca domestica] | 0.0 | Serine protease inhibitor 3/4-like | MdSerpin 3 |
| CL8948.Contig3 | 1508 | Serpin(65-419) | — | XP_005177808.1 [Musca domestica] | 0.0 | Serine protease inhibitor 3/4-like | MdSerpin 3 |
| Unigene47907 | 1122 | Serpin(1-334) | — | XP_005177808.1 [Musca domestica] | 2e-147 | Serine protease inhibitor 3/4-like | MdSerpin 3 |
| Unigene3626 | 923 | Serpin(38-291) | 1-24 | XP_005177808.1 [Musca domestica] | 2e-88 | Serine protease inhibitor 3/4-like | MdSerpin 4 |
| CL9667.Contig1 | 1565 | Serpin(52-422) | 1-20 | ABC25075.1 [Glossina morsitans morsitans] | 0.0 | Serine protease inhibitor 5 | MdSerpin 5 |
| Unigene2774 | 1548 | Serpin(87-450) | 1-23 | XP_005183089.1 [Musca domestica] | 0.0 | Serpin B3-like | MdSerpin 6 |
| Unigene40627 | 1248 | Serpin(32-378) | 1-20 | XP_005179838.1 [Musca domestica] | 0.0 | Serine protease inhibitor 3/4-like isoform X2 | MdSerpin 6 |
| CL11280.Contig1 | 759 | Serpin(1-149) | — | XP_005187409.1 [Musca domestica] | 2e-64 | Leukocyte elastase inhibitor-like | MdSerpin 6 |
| CL10810.Contig2 | 1189 | Serpin(24-163) | 1-20 | XP_004533758.1 [Ceratitis capitata] | 3e-72 | Serpin B4-like | MdSerpin 7 |
| Unigene49496 | 1670 | Serpin(66-437) | 1-23 | XP_005183496.1 [Musca domestica] | 0.0 | Plasminogen activator inhibitor 2-like | MdSerpin 8 |
| CL13613.Contig2 | 774 | Serpin(1-182) | — | XP_005183496.1 [Musca domestica] | 2e-66 | Plasminogen activator inhibitor 2-like | MdSerpin 8 |
| Unigene42869 | 732 | Serpin(1-184) | — | ABC25079.1 [Glossina morsitans morsitans] | 6e-61 | Serine protease inhibitor | MdSerpin 8 |
| Unigene45821 | 777 | Serpin(17-237) | — | XP_005185733.1 [Musca domestica] | 2e-170 | Leukocyte elastase inhibitor-like | MdSerpin 9 |
| CL1905.Contig2 | 1942 | Serpin(75-622) | 1-27 | XP_005188485.1 [Musca domestica] | 4e-121 | Leukocyte elastase inhibitor B-like | MdSerpin 9 |
| CL530.Contig1 | 1604 | Serpin(49-418) | 1-19 | XP_005191108.1 [Musca domestica] | 0.0 | Serpin B6-like | MdSerpin 10 |
| CL7972.Contig1 | 802 | Serpin(1-215) | — | XP_005182902.1 [Musca domestica] | 4e-107 | Uncharacterized protein LOC101899860 | MdSerpin 11 |
| Unigene32587 | 735 | Serpin(1-217) | — | XP_005182902.1 [Musca domestica] | 4e-163 | Uncharacterized protein LOC101899860 | MdSerpin 11 |
| Unigene34684 | 750 | Serpin(1-233) | — | XP_005188485.1 [Musca domestica] | 7e-95 | Leukocyte elastase inhibitor B-like | MdSerpin 11 |
| Unigene1407 | 1017 | Serpin(1-302) | — | XP_005183089.1 [Musca domestica] | 0.0 | serpin B3-like | MdSerpin 11 |
| Unigene14891 | 1116 | Serpin(85-351) | 1-29 | XP_005183089.1 [Musca domestica] | 0.0 | Serpin B3-like | MdSerpin 11 |
| Unigene9084 | 476 | Serpin(1-145) | — | XP_005185733.1 [Musca domestica] | 4e-57 | Leukocyte elastase inhibitor-like | MdSerpin 11 |
a
The protein domains and bsignal peptide are predicted by available nucleotide sequences, respectively.
c
The putative genes were provided mainly with reference to unigene annotation and unigene phylogenetic analysis (see Figure 5, Figure 6, Figure 7, Figure 8, and Figure S1).
d
The number indicates the length of splicing nucleotide sequences of Unigene35644 and Unigene35645.