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. 2015 Jun 23;6:2041731415592356. doi: 10.1177/2041731415592356

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© The Author(s) 2015

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (http://www.uk.sagepub.com/aboutus/openaccess.htm).

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Figure 3. — Osteogenic and adipogenic differentiation analysed by (1) alizarin red (AR) and (2) Oil Red-O (ORO) staining, respectively. AR staining and quantification were used to identify calcium deposition. ORO staining and quantification were used to identify lipid accumulation. For both assays, CD29⁺/CD90⁺, unlabelled and unsorted (a) ADC and (b) BMDC cultures were compared. Both analyses were performed following a 21-day culture in osteogenic or adipogenic medium.

*p < 0.05, n = 3.