Table 1.
Passage 2 | Passage 3 | Passage 4 | Passage 5 | Passage 6 | Passage 7 (in scaffold) | Condition | |
---|---|---|---|---|---|---|---|
NCCs | 10% FBS | Pellet | X | X | X | X | Passage 2 in 10% FBS |
2.5% PRPr | X | X | X | X | Passage 2 in 2.5% PRPr | ||
10% PRPr | X | X | X | X | Passage 2 in 10% PRPr | ||
10% FBS | Pellet | X | X | Passages 2 to 4 in 10% FBS | |||
2.5% PRPr | X | X | Passages 2 to 4 in 2.5% PRPr | ||||
10% PRPr | X | X | Passages 2 to 4 in 10% PRPr | ||||
10% FBS | 2.5% PRPr | X | X | Passage 4 in 2.5% PRPr | |||
10% FBS | 10% PRPr | X | X | Passage 4 in 10% PRPr | |||
MSCs | 10% FBS | 2.5% PRPr | 2.5% PRPr | ||||
10% FBS | 10% PRPr | 10% PRPr | |||||
10% FBS | CM + 2.5% PRPr | CM + 2.5% PRPr | |||||
10% FBS | CM + 10% PRPr | CM + 10% PRPr | |||||
10% FBS | 2.5% PRPr | CM + 10% FBS | CM + 10% FBS_PRPr exp | ||||
10% FBS | CM + 10% FBS | CM + 10% FBS_FBS exp |
NCCs: nasoseptal chondrogenic cells; MSCs: mesenchymal stromal cells; FBS: fetal bovine serum; PRPr: platelet-rich plasma releasate; CM: chondrogenic medium.
All culture conditions summarized for NCCs and MSCs. Passages where no culture or experiment was performed on the determined condition are marked with an X symbol. Light green shade corresponds to 10% FBS treatment, yellow to 2.5% PRPr, light blue to 10% PRPr, orange to CM + 2.5% PRPr, dark blue to CM + 10% PRPr, and dark green to CM + 10% FBS.