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. 2015 Sep;146(3):205–219. doi: 10.1085/jgp.201511383

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© 2015 López Soto et al.

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Figure 5. — GHSR1a activity modulates native Ca_V2 currents in hypothalamic neurons from GHSR-eGFP reporter mice. (A) Representative I_Ba traces and averaged I_Ba before (control) and after (+ghrelin) 500-nM ghrelin application in hypothalamic GHSR1a− and GHSR1a+ neurons. (B) Averaged peak I_Ba–voltage (I-V) relationships (evoked from a holding of −80 mV), reversal (V_rev), and activation (V_1/2) potential midpoints (calculated by Boltzmann linear function) obtained from GHSR1a− and GHSR1a+ neurons. (C) I_Ba time courses of application of 1 µM ω-conotoxin-GVIA (conoTx) and 0.2 µM ω-agatoxin-IVA (agaTx) with or without previous 500-nM ghrelin application from hypothalamic GHSR1a− (top) and GHSR1a+ neurons (middle and bottom; left). Averaged percentage of I_Ba sensitive to agaTx and conoTx from GHSR1a− and GHSR1a+ neurons, with (+ghrelin) or without 500-nM ghrelin application (right). (D) Representative and averaged I_Na from GHSR1a− and GHSR1a+ neurons. Paired (A) or two-sample (B and D) Student’s t test and ANOVA with Dunnett’s post-test (C). *, P < 0.05. Error bars represent mean ± SE.