Figure 4. BUD1 deletion has no effect on inheritance of the ER and CPY* aggregates.
(A) cER inheritance is blocked to similar extents in WT and bud1Δ cells upon ER stress induction (1 μg/ml Tm for 3 hr). (B) bud1Δ cells display a normal block in ER inheritance upon exposure to ER stress induced by CPY*-mRFP expression for 2 hr. (C) Percentage of bud1Δ daughter cells containing CPY*-mRFP aggregates in class I, II, and III cells. (D) Distribution (%) of cells at different stages of the cell cycle in which daughter cells contain cER and CPY*-mRFP aggregates (yellow), cER but not CPY*-mRFP aggregates (gray), and neither cER nor CPY*-mRFP aggregates (light blue). (E) Distribution of CPY*-mRFP aggregates per bud in all, class I, and class II + III bud1Δ cells.