FIG. 1.

E7 domain directs greater loading of BMP2pep onto ABB particles. (A) ABB particles were incubated with equimolar concentrations of the FITC-tagged peptides (either 10 or 100 μM) for 30 min, and then samples were washed with TBS. Increased binding of E7BMP2pep, relative to BMP2pep, was apparent on the particles by the naked eye (yellow tint). (B) ABB particles were conjugated with E7BMP2pep-FITC, BMP2pep-FITC (both peptides at 10 μM), or with saline (no peptide control) for 30 min. Imaging of samples using a fluorescent microscope revealed greater binding of E7BMP2pep compared with BMP2pep. ABB, anorganic bovine bone; BMP2, bone morphogenic protein 2; FITC, fluorescein isothiocyanate; TBS, tris-buffered saline. Color images available online at www.liebertpub.com/tea