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Bcl-2 is a direct target of miR-497. A. The miR-497 binding site in the 3’-UTR of Bcl-2 mRNA and the Bcl-2 3’-UTR mutant sequence. B. The relative luciferase activity (Firefly/Renilla) was measured in HEK293T cells after co-transfection of the Bcl-2 3’-UTR luciferase construct with miR-497 mimics or miR-NC and co-transfection of the Bcl-2 3’-UTR mutant luciferase construct with miR-497 mimics or miR-NC.
