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Primers used for the quantitative real-time PCR
| Gene Name | Primer Sequence (5’ to 3’) | |
|---|---|---|
| C-myc | Forward | TCAAGAGGCGAACACACAAC |
| Reverse | GGCCTTTTCATTGTTTTCCA | |
| cyclin D1 | Forward | ACCTGAGGAGCCCCAACAA |
| Reverse | TCTGCTCCTGGCAGGCC | |
| β-catenin | Forward | GCAGCAACAGTCTTACCT |
| Reverse | ACAGGACTTGGGAGGTAT | |
| LEF-1 | Forward | CTTCGCCGAGATCAGTCA |
| Reverse | CTGGCCTTGTCGTGGTAG | |
| β-actin | Forward | TCCATCATGAAGTGTGACGT |
| Reverse | GAGCAATGATCTTGATCTTCAT | |
