Immunohistochemical findings for GFAP (A). S-100 protein (B). In the astrocytoma cell portion. Immunohistochemical findings for GFAP (C), nestin (D), MAP-2(E), EMA (F) in the anaplastic ependymoma cell component. The mesenchymal component cells were strongly positive for reticulin staining (G), Immunostaining for GFAP (H), nestin (I), S-100 (J) and Olig-2 (K) were not detected. Also all of the specific mesenchymal markers (SMA, desmin, α-sarcomeric) were not immunoreactive. INI-1 was positive in both components (L).