Figure 3.
Effects of NAC on CCl4-induced hepatic Nrf2/HO-1 pathway. A. Nrf2, HO-1 and GAPDH mRNA expression in rat livers. B. Assessment of the results was done by measuring the levels of Nrf2, HO-1 and GAPDH mRNA expression. I, The control group; II, The CCl4-induced group; III, 50 mg/kg NAC treatment group; IV, 100 mg/kg NAC treatment group; V, 200 mg/kg NAC treatment group. The results were analyzed using one-way analysis of variance and Dunnett’s test and the data were expressed as means ± standard error of the mean of 3 mice in each group. *P<0.05, vs. the control group, #P<0.05, vs. the CCl4 group, as determined by one-way analysis of variance and Dunnett’s test. NAC, N-acetylcysteine; CCl4, carbon tetrachloride; Nrf2, nuclear factor erythroid 2-related factor 2; HO-1, heme oxygenase-1; GAPDH, glyceraldehydes phosphate dehydrogenase.