Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Aug 31;210(5):717–726. doi: 10.1083/jcb.201501053

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Parisi et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 2. — APC is required for adult skeletal muscle regeneration. (A) TM regimen and cardiotoxin (CTX) injury scheme for control and APC SC-KO mice. (B and C) Immunostaining for Pax7 (B) and e-MyHC (C) on TA cryosections 4 d after injury. (D–L) Analysis of TA muscles 14 d after injury reveals that tissue regeneration is defective in APC SC-KO muscles. (D) Whole sections of TA muscles immunostained for Laminin. Immunostaining for Tcf4 (F), type 1 Collagen (H), and Pax7 and Laminin (L) on TA cryosections is shown. Quantification of TA weight 14 d after injury normalized by uninjured TA weight (E), of the number of Tcf4+ cells/mm² (G), of relative Collagen+ area (I), and of sublaminar Pax7+ cells (normalized by Pax7+ cells of uninjured TA; J) is also shown. (K) GFP immunostaining on transverse sections of TAs isolated from Pax7^CreER;Z/EG (Control-Z/EG) and Pax7^CreERAPC^lox/lox;Z/EG (APC SC-KO-Z/EG) 14 d after injury demonstrates that APC-null satellite cells do not give rise to new myofibers, nor trans-differentiate in other cell types. Bars: (B, C, F, H, K, and L) 50 µm; (D) 200 µm. Nuclei are stained with Hoechst. Error bars indicate SEM. Inset panels show a 2× enlargement.