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. 2015 Aug 31;210(5):801–816. doi: 10.1083/jcb.201501076

Figure 2.

Figure 2.

Dynamin-mediated endocytosis is required for wound closure. (A and B) Wound closure in embryos expressing E-cadherin:GFP (green) and myosin:mCherry (red), injected with DMSO (A) or dynasore (B) before wounding. (C and D) Mean wound area over time (C) and closure rate for the fast phase of wound repair (D) for control (blue; n = 12) and dynasore-injected embryos (red; n = 11). (E–G) Wound closure in wild-type embryos expressing GFP:moesin and heated to the restrictive temperature (35°C; E), or carrying a temperature-sensitive allele of dynamin (shits1) at the permissive temperature (23°C; F) or the restrictive temperature (G). (H and I) Mean wound area over time (H) and closure rate for the fast phase of wound repair (I) for wild-type embryos at the restrictive temperature (blue; n = 5), shits1 embryos at the permissive temperature (green; n = 6), or shits1 embryos at the restrictive temperature (red; n = 6). (A, B, and E–G) Time after wounding is shown. Red lines indicate wound sites. Yellow dotted lines outline the wounds. Anterior left, dorsal up. Bars, 5 µm. Error bars, SEM; *, P < 0.05; ***, P < 0.001.